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چه طول موج لیزر برای طیف سنجی رامان استفاده می شود؟

چه طول موج لیزر برای طیف سنجی رامان استفاده می شود؟

طول موج لیزر اعم از اشعه ماوراء بنفش از طریق به نزدیکی مادون قرمز قابل مشاهده را می توان برای طیف سنجی رامان استفاده می شود.نمونه های معمولی شامل (اما نه محدود به) :

از اشعه ماوراء بنفش: 244 نانومتر، 257 نانومتر، 325 نانومتر، 364 نانومتر
قابل مشاهده: 457 نانومتر، 473 نانومتر، 488 نانومتر، 514 نانومتر، 532 نانومتر، 633 نانومتر، 660 نانومتر
به مادون قرمز نزدیک است : 785 نانومتر، 830 نانومتر، 980 نانومتر، 1064 نانومتر

انتخاب طول موج لیزر است تاثیر مهمی در قابلیت های تجربی :

حساسیت. شدت پراش رامان متناسب با λ λ-4 که در آن طول موج لیزر است. بنابراین مادون قرمز نتایج لیزر در کاهش پراکندگی شدت توسط یک عامل از 15 یا بیشتر، زمانی که با رنگ آبی / سبز لیزر قابل مشاهده مقایسه شده است.

رزولوشن فضایی.پراش محدود لیزر قطر نقطه می توان با توجه به معادله، قطر محاسبه = 1.22 λ / NA (که در آن λ طول موج لیزر است، و NA دیافراگم عددی هدف میکروسکوپ استفاده می شود). به عنوان مثال، با یک لیزر نانومتر 532، و هدف 0.90 / 100X، قطر نقطه نظری خواهد بود .72 میکرومتر - با هدف مشابه، یک لیزر 785 نانومتر یک قطر نقطه نظری 1.1 میکرومتر داشت. بنابراین، قدرت تفکیک مکانی دست یافتنی نیمه وابسته به انتخاب لیزر است.

بهینه سازی حاصله بر رفتار نمونه است. برای مثال:
آبی یا لیزر سبز می تواند خوب باشد برای مواد معدنی و رزونانس آزمایش رامان (به عنوان مثال، برای نانولوله های کربنی و دیگر مواد کربن) و پراکندگی سطحی افزایش یافته است رامان (SERS) است .

قرمز یا نزدیک مادون قرمز (660-830 نانومتر) خوب برای سرکوب فلورسانس می باشد.

لیزر فرابنفش برای رامان رزونانس در زیستی مولکول (مانند پروتئین ها، DNA، RNA و)، و سرکوب فلورسانس است .






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